Molecular Diagnostics

Infectious Pancreatic Necrosis Virus (IPNV), Lyophilized RT-qPCR

Overview

Infectious Pancreatic Necrosis (IPN) is a highly contagious viral disease affecting primarily young salmonids (such as Atlantic salmon and Rainbow trout) during the fry and post-smolt stages, though adult fish may carry the virus asymptomatically. The causative agent IPNV is a bi-segmented double-stranded RNA virus (Family Birnaviridae) that is extremely stable in the aquatic environment and capable of both vertical (egg) and horizontal transmission. Clinically, infected fish can present darkened skin, pale gills, abdominal distension (ascites), and high mortality, especially in younger fish; necrosis of the pancreas is a hallmark on histology. In aquaculture, IPN causes significant economic losses through mortality, reduced growth and latent carrier populations that facilitate further outbreaks.

The Infectious Pancreatic Necrosis Virus (IPNV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of IPNV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of IPNV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of IPNV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Infectious Pancreatic Necrosis (IPN))
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Salmonid Alphavirus (SAV), Lyophilized RT-qPCR

Overview

Salmonid Alphavirus (SAV) is an enveloped, positive-sense RNA virus (Family Togaviridae, genus Alphavirus) responsible for the disease commonly called Pancreas Disease (PD) in Atlantic salmon and “Sleeping Disease” in rainbow trout. It is particularly important in European aquaculture, with different genotypes (SAV1-6) causing outbreaks. Clinically, the disease involves inflammation and necrosis of the pancreas, heart and skeletal muscle leading to reduced feed intake, lethargy, growth depression and sometimes increased mortality.

The Salmonid Alphavirus (SAV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of SAV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of SAV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of SAV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation 
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Piscine Myocarditis Virus (PMCV), Lyophilized RT-qPCR

Overview

Piscine Myocarditis Virus (PMCV) is the causative agent of Cardiomyopathy Syndrome (CMS) in farmed Atlantic salmon, especially in the marine grow-out phase. PMCV is a double-stranded RNA virus (provisionally of the Totiviridae family) that causes severe inflammation and necrosis of the heart muscle (ventricle) leading to ascites (fluid accumulation), sudden mortality, or chronic loss of condition. CMS often appears late in the production cycle (typically second year at sea) and presents a major economic challenge due to large fish biomass and limited effective treatments.

The Piscine Myocarditis Virus (PMCV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of PMCV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of PMCV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of PMCV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Cardiomyopathy Syndrome (CMS))
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Infectious Salmon Anaemia Virus (ISAV), Lyophilized RT-qPCR

Overview

Infectious Salmon Anaemia (ISA) is a serious, immunologically and economically significant disease caused by the orthomyxovirus ISAV in Atlantic salmon, and occasionally in other salmonids. The virus can induce severe anaemia, hemorrhages (internal and external), ascites, swollen liver/spleen, and high mortalities in marine cage production. Because of its high risk, ISA is a notifiable disease in many jurisdictions.

The Infectious Salmon Anaemia Virus (ISAV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of ISAV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of ISAV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of ISAV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Infectious Salmon Anaemia (ISA))
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Piscine Orthoreovirus (PRV), Lyophilized RT-qPCR

Overview

Piscine Orthoreovirus (PRV) is a dsRNA virus (Family Reoviridae, genus Orthoreovirus) that infects salmonids (e.g., Atlantic salmon, rainbow trout) and is associated with Heart and Skeletal Muscle Inflammation (HSMI) and other conditions. In Atlantic salmon, PRV infection commonly occurs post seawater-transfer and may lead to inflammation of heart and skeletal muscle, reduced condition and in some cases mortality (up to ~20 %). However, many infected fish carry PRV without clinical disease, and the relationship between PRV presence and disease severity is a topic of ongoing research.

The Piscine Orthoreovirus (PRV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of PRV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of PRV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of PRV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Heart and Skeletal Muscle Inflammation (HSMI))
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Infectious Hematopoietic Necrosis Virus (IHNV), Lyophilized RT-qPCR

Overview

Infectious Hematopoietic Necrosis Virus (IHNV) is a pathogenic rhabdovirus (Family Rhabdoviridae, genus Novirhabdovirus) affecting salmonid fry and juveniles in freshwater and early marine phases. Infected fish may show darkened colour, exophthalmia (bulging eyes), pale gills (anaemia), hemorrhages on skin and fins, and high mortality, especially under stress or in dense rearing conditions. Because young fish are most susceptible, IHNV remains a key concern in hatcheries and early sea-transfer phases.

The Infectious Hematopoietic Necrosis Virus (IHNV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of IHNV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of IHNV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of IHNV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Infectious Hematopoietic Necrosis)
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Salmon Gill Poxvirus (SGPV), Lyophilized qPCR

Overview

Salmon Gill Poxvirus (SGPV) is a large DNA virus that specifically infects the gills of Atlantic salmon, causing Salmon Gill Poxvirus Disease (SGPVD). Clinical signs include severe gill epithelial cell necrosis/hyperplasia, respiratory distress, lethargy, and elevated mortalities in smolt or seawater-transferred salmon. This pathogen poses an increasing concern in aquaculture as it directly impacts the gill function and thus overall performance and welfare of farmed salmon.

The Salmon Gill Poxvirus (SGPV) Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of SGPV DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of SGPV DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of SGPV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Salmon Gill Poxvirus Disease (SGPVD))
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Viral Hemorrhagic Septicemia Virus (VHSV), Lyophilized RT-qPCR

Overview

Viral Hemorrhagic Septicemia (VHS), caused by VHSV (genus Novirhabdovirus, family Rhabdoviridae), is one of the most severe viral diseases in fish globally, affecting more than 80 (even 140+) species in freshwater and marine systems. The disease features haemorrhages in internal organs, muscle and skin, abnormal swimming behaviour, darkening of the body, exophthalmia and sudden mass mortalities. The broad host range and environmental persistence make VHSV a major biosecurity threat in aquaculture and wild fisheries.

The Viral Hemorrhagic Septicemia Virus (VHSV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of VHSV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of VHSV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of VHSV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Viral Hemorrhagic Septicemia)
• Research use in virology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Yersinia ruckeri, Lyophilized qPCR

Overview

Yersinia ruckeri is a Gram-negative bacterium causing Enteric Redmouth Disease (ERM) in salmonids such as rainbow trout and occasionally Atlantic salmon. Characteristic signs include haemorrhages in the mouth, eyes (exophthalmia), darkened skin, lethargy and internal bleeding. The disease remains of commercial importance in freshwater and early grow-out phases, and is managed with vaccines in many regions.

The Yersinia ruckeri Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of Y. ruckeri DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of Y. ruckeri DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of Y. ruckeri in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Enteric Redmouth Disease (ERM))
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Renibacterium salmoninarum, Lyophilized qPCR

Overview

Renibacterium salmoninarum is the Gram-positive intracellular bacterium responsible for Bacterial Kidney Disease (BKD) in salmonids. The infection is often chronic and insidious, affecting older juveniles and adults, with signs such as white granulomas in the kidney and spleen, anemia, reduced growth and low-level mortalities rather than sudden outbreaks. Because fish may carry the bacterium subclinically, it presents a persistent challenge in broodstock and grow-out operations.

The Renibacterium salmoninarum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of R. salmoninarum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of R. salmoninarum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of R. salmoninarum in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Bacterial Kidney Disease (BKD))
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Aeromonas salmonicida, Lyophilized qPCR

Overview

Aeromonas salmonicida is a Gram-negative rod that causes furunculosis—a systemic disease in salmonids. The disease features external signs such as ulcers (“furuncles”), hemorrhages, darkened skin, distended abdomen, and internal signs like swollen kidney/spleen, and haemorrhagic septicemia. Although historically devastating, many aquaculture systems now manage furunculosis with vaccinations and improved husbandry.

The Aeromonas salmonicida Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of A. salmonicida DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of A. salmonicida DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of A. salmonicida in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Furunculosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Flavobacterium psychrophilum, Lyophilized qPCR

Overview

Flavobacterium psychrophilum is a Gram-negative psychrophilic bacterium causing Bacterial Cold Water Disease (BCWD) and Rainbow Trout Fry Syndrome in many salmonids. The disease chiefly affects young fish in freshwater (temperatures <15 °C), where lesions such as fin erosion, skin ulceration, mouth deformities, and internal organ damage lead to high mortality and chronic losses. Vertical and horizontal transmission have been documented, and controlling this pathogen remains challenging in hatchery settings.

The Flavobacterium psychrophilum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of F. psychrophilum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of F. psychrophilum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of F. psychrophilum in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Bacterial Cold Water Disease (BCWD))
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Neoparamoeba perurans (Amoebic Gill Disease, AGD), Lyophilized qPCR

Overview

Neoparamoeba perurans is a free-living marine amoeba that causes Amoebic Gill Disease (AGD) in farmed marine salmonids (especially Atlantic salmon). AGD is characterised by white mucoid patches on the gills, gill epithelial hyperplasia and fusion, respiratory distress, lethargy and reduced growth. Environmental factors such as higher water temperature and crowding influence the outbreak risk.

The Neoparamoeba perurans Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of N. perurans DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of amoeba load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of N. perurans DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of N. perurans in fish tissue, gill swabs, or environmental water samples
• Aquaculture health monitoring and outbreak investigation (e.g., Amoebic Gill Disease (AGD))
• Research use in parasitology, epidemiology, and treatment efficacy studies

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Piscirickettsia salmonis, Lyophilized qPCR

Overview

Piscirickettsia salmonis is a facultative intracellular Gram-negative bacterium that causes Piscirickettsiosis (also called Salmon Rickettsial Septicaemia, SRS) in farmed salmonids, especially Atlantic and Coho salmon. The disease typically occurs in seawater grow-out and is characterised by lethargy, reduced appetite, skin lesions/ulcers, kidney damage, anemia and high mortality (sometimes up to ~90% in extremes). Management is complicated by the intracellular nature of the pathogen, variable vaccine efficacy and environmental persistence.

The Piscirickettsia salmonis Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of P. salmonis DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of P. salmonis DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of P. salmonis in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Piscirickettsiosis / SRS)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Renibacterium salmoninarum, Lyophilized qPCR

Overview

Renibacterium salmoninarum is the Gram-positive intracellular bacterium responsible for Bacterial Kidney Disease (BKD) in salmonids. The infection is often chronic and insidious, affecting older juveniles and adults, with signs such as white granulomas in the kidney and spleen, anemia, reduced growth and low-level mortalities rather than sudden outbreaks. Because fish may carry the bacterium subclinically, it presents a persistent challenge in broodstock and grow-out operations.

The Renibacterium salmoninarum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of R. salmoninarum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of R. salmoninarum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of R. salmoninarum in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Bacterial Kidney Disease (BKD))
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Lactococcus garvieae, Lyophilized qPCR

Overview

Lactococcus garvieae is a Gram-positive coccus that causes lactococcosis, typically a haemorrhagic septicemia in fish, and is emerging in marine species including sea bass (rarely reported) and trout. When infection occurs in grow-out stages, clinical signs may include haemorrhages, exophthalmia (bulging eyes), abnormal swimming behaviour, lethargy and high mortality in some cases. Commercial vaccines exist for trout (fresh‐water salmonids) and are under development for marine species such as sea bass.

The Lactococcus garvieae Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of L. garvieae DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of L. garvieae DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of L. garvieae in fish tissue, internal organs, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Lactococcosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Sparicotyle chrysophrii, Lyophilized qPCR

Overview

Sparicotyle chrysophrii is a gill ectoparasitic monogenean (family Microcotylidae) that infects gilthead sea bream and to a lesser extent sea bass, especially during the grow-out phase. All life stages can be affected, though the parasite load and gill damage tend to accumulate in larger fish. Clinically, infections lead to anemia, severe gill lamellar damage (shortening, fusion of secondary lamellae), respiratory distress, lethargy, and reduced growth performance. There is currently no commercial vaccine, and control relies on regular monitoring and bath treatments.

The Sparicotyle chrysophrii Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of S. chrysophrii DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of parasite load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of S. chrysophrii DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of S. chrysophrii in fish tissue (gills), gill swabs, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., gill monogenean infestations)
• Research use in parasitology, epidemiology, and treatment efficacy studies

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Vibrio harveyi, Lyophilized qPCR

Overview

Vibrio harveyi is a motile, Gram-negative marine bacterium implicated in vibriosis of fish and invertebrates. In sea bass and sea bream, it can infect at all life-stages and is often associated with skin lesions, fin necrosis, eye opacity, lethargy, and haemorrhages under stress-prone, warm‐water conditions. No widely deployed commercial vaccine exists specifically for V. harveyi in Mediterranean sea bass/sea bream farming.

The Vibrio harveyi Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of V. harveyi DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of V. harveyi DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of V. harveyi in fish tissue, skin lesions, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Vibriosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Photobacterium damselae, Lyophilized qPCR

Overview

Photobacterium damselae is a halophilic, Gram-negative rod that causes photobacteriosis (also called fish pasteurellosis) in marine species including sea bass and sea bream. It commonly affects juveniles and adults, with outbreaks often linked to elevated water temperature (>18–20 °C). Clinically one sees fish with abdominal distension/ascites, external haemorrhages, skin ulcers, and internal nodules or necrotic foci in organs such as liver, spleen and kidney.

The Photobacterium damselae Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of P. damselae DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of P. damselae DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of P. damselae in fish tissue, skin lesions, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Photobacteriosis / Fish Pasteurellosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.

Tenacibaculum maritimum, Lyophilized qPCR

Overview

Tenacibaculum maritimum is a Gram-negative, filamentous marine bacterium that causes the disease known as tenacibaculosis in many cultured marine fish species including sea bass (European sea bass) and sea bream (Gilthead sea bream). It affects all life stages, though juveniles are more susceptible (weights ~2-80 g) under conditions of stress, elevated temperature (>15 °C) and high salinity. The infection typically begins with pale foci and yellow-bordered lesions on skin/mouth/fin bases that rapidly progress into deep necrotic ulcers, fin and tail erosion, and in severe cases mortality.

The Tenacibaculum maritimum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of T. maritimum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of T. maritimum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of T. maritimum in fish tissue, skin lesions, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Tenacibaculosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Note: For research use only. Not intended for diagnostic or therapeutic applications.