Molecular Diagnostics

Flavobacterium psychrophilum, Lyophilized qPCR

Overview

Flavobacterium psychrophilum is a Gram-negative psychrophilic bacterium causing Bacterial Cold Water Disease (BCWD) and Rainbow Trout Fry Syndrome in many salmonids. The disease chiefly affects young fish in freshwater (temperatures <15 °C), where lesions such as fin erosion, skin ulceration, mouth deformities, and internal organ damage lead to high mortality and chronic losses. Vertical and horizontal transmission have been documented, and controlling this pathogen remains challenging in hatchery settings.

The Flavobacterium psychrophilum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of F. psychrophilum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of F. psychrophilum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of F. psychrophilum in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Bacterial Cold Water Disease (BCWD))
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Parameter	Description
Detection method	Probe-based real-time qPCR
Target	Flavobacterium psychrophilum DNA
Format	Lyophilized (single-reaction beads)
Storage temperature	Room temperature
Shelf life	12 months
Intended use	For research use only

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Product	Cat. No.
F. psychrophilum qPCR Kit (Lyophilized)	F.PSY-LYO-01

Note: For research use only. Not intended for diagnostic or therapeutic applications.