Molecular Diagnostics

Viral Hemorrhagic Septicemia Virus (VHSV), Lyophilized RT-qPCR

Overview

Viral Hemorrhagic Septicemia (VHS), caused by VHSV (genus Novirhabdovirus, family Rhabdoviridae), is one of the most severe viral diseases in fish globally, affecting more than 80 (even 140+) species in freshwater and marine systems. The disease features haemorrhages in internal organs, muscle and skin, abnormal swimming behaviour, darkening of the body, exophthalmia and sudden mass mortalities. The broad host range and environmental persistence make VHSV a major biosecurity threat in aquaculture and wild fisheries.

The Viral Hemorrhagic Septicemia Virus (VHSV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of VHSV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of VHSV RNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of VHSV in fish tissue, cell culture, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Viral Hemorrhagic Septicemia)
• Research use in virology, epidemiology, and vaccine development

Specifications

Parameter	Description
Detection method	Probe-based real-time RT-qPCR
Target	Viral Hemorrhagic Septicemia Virus (VHSV) RNA
Format	Lyophilized (single-reaction beads)
Storage temperature	Room temperature
Shelf life	12 months
Intended use	For research use only

Package Contents

• Lyophilized RT-qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add RNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Product	Cat. No.
VHSV RT-qPCR Kit (Lyophilized)	VHSV-LYO-01

Note: For research use only. Not intended for diagnostic or therapeutic applications.