Molecular Diagnostics

Tenacibaculum maritimum, Lyophilized qPCR

Overview

Tenacibaculum maritimum is a Gram-negative, filamentous marine bacterium that causes the disease known as tenacibaculosis in many cultured marine fish species including sea bass (European sea bass) and sea bream (Gilthead sea bream). It affects all life stages, though juveniles are more susceptible (weights ~2-80 g) under conditions of stress, elevated temperature (>15 °C) and high salinity. The infection typically begins with pale foci and yellow-bordered lesions on skin/mouth/fin bases that rapidly progress into deep necrotic ulcers, fin and tail erosion, and in severe cases mortality.

The Tenacibaculum maritimum Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of T. maritimum DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of bacterial load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of T. maritimum DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of T. maritimum in fish tissue, skin lesions, or environmental samples
• Aquaculture health monitoring and outbreak investigation (e.g., Tenacibaculosis)
• Research use in microbiology, epidemiology, and vaccine development

Specifications

Parameter	Description
Detection method	Probe-based real-time qPCR
Target	Tenacibaculum maritimum DNA
Format	Lyophilized (single-reaction beads)
Storage temperature	Room temperature
Shelf life	12 months
Intended use	For research use only

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Product	Cat. No.
T. maritimum qPCR Kit (Lyophilized)	T.MAR-LYO-01

Note: For research use only. Not intended for diagnostic or therapeutic applications.