Molecular Diagnostics

Neoparamoeba perurans (Amoebic Gill Disease, AGD), Lyophilized qPCR

Overview

Neoparamoeba perurans is a free-living marine amoeba that causes Amoebic Gill Disease (AGD) in farmed marine salmonids (especially Atlantic salmon). AGD is characterised by white mucoid patches on the gills, gill epithelial hyperplasia and fusion, respiratory distress, lethargy and reduced growth. Environmental factors such as higher water temperature and crowding influence the outbreak risk.

The Neoparamoeba perurans Probe-based qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of N. perurans DNA in biological samples.
This assay utilizes a probe-based real-time qPCR approach to ensure high specificity and accurate quantification of amoeba load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.

Each lyophilized reaction bead is pre-formulated with all essential components — DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.

Key Features

• Specific and Sensitive: Designed for accurate detection of N. perurans DNA using TaqMan-style probe chemistry.
• Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and DNA template.
• Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
• Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
• Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.

Applications

• Detection and quantification of N. perurans in fish tissue, gill swabs, or environmental water samples
• Aquaculture health monitoring and outbreak investigation (e.g., Amoebic Gill Disease (AGD))
• Research use in parasitology, epidemiology, and treatment efficacy studies

Specifications

Parameter	Description
Detection method	Probe-based real-time qPCR
Target	Neoparamoeba perurans DNA
Format	Lyophilized (single-reaction beads)
Storage temperature	Room temperature
Shelf life	12 months
Intended use	For research use only

Package Contents

• Lyophilized qPCR reaction beads (pre-aliquoted)
• Nuclease-free rehydration buffer (optional)
• Product manual with detailed instructions

Instructions for Use

1. Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
2. Add DNA template (extracted from sample).
3. Run on a compatible real-time PCR instrument using the supplied cycling conditions.
4. Analyze amplification curves for quantification and interpretation.

Ordering Information

Product	Cat. No.
N. perurans qPCR Kit (Lyophilized)	N.PER-LYO-01

Note: For research use only. Not intended for diagnostic or therapeutic applications.