Product Name: Vimentin (phospho Ser56) Polyclonal Antibody
Catalog No.: ALP0767
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: VIM
Protein Name: Vimentin
Human Gene ID: 7431
Human Swiss Prot No.: P08670
Other Name: VIM; Vimentin
Subcellular Location: Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane .
Expression: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt’s lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser56) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser56) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from A549 cells treated with Nocodazole 1ug/ml 16h, using Vimentin (Phospho-Ser56) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.