Product Name: Ub Polyclonal Antibody

Catalog No.: ALT5541

Reactivity: Human;Mouse;Rat

Applications: IF/ICC;WB;IHC-p;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF: 1:50-200 Western Blot: 1/500 – 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: UBA52/RPS27A/UBB/UBC

Protein Name: Ubiquitin

Human Gene ID:

Human Swiss Prot No.: P62987/P62979/P0CG47/P0CG48

Other Name: UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1

Subcellular Location: [Ubiquitin]: Cytoplasm . Nucleus .; [60S ribosomal protein L40]: Cytoplasm .

Expression: Brain,Epithelium,Fetal brain cortex,Liver,L

Immunofluorescence analysis of human-lung tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of K562, L929 cells using Ub Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunofluorescence analysis of rat-kidney tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100

Immunofluorescence analysis of rat-kidney tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Ub Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of mouse-spleen tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

For research use only. Not for use in diagnostic procedures.

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