Product Name: TNAP Polyclonal Antibody

Catalog No.: ALT5563

Reactivity: Human;Rat

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: ALPL

Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme

Human Gene ID: 249

Human Swiss Prot No.: P05186

Other Name: ALPL; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme

Subcellular Location: Cell membrane ; Lipid-anchor, GPI-anchor . Extracellular vesicle membrane ; Lipid-anchor, GPI-anchor . Mitochondrion membrane ; Lipid-anchor, GPI-anchor . Mitochondrion intermembrane space . Localizes to special class of extracellular vesicles, named matrix vesicles (MVs), which are released by osteogenic cells. Localizes to the mitochondria of thermogenic fat cells: tethered to mitochondrial membranes via a GPI-anchor and probably resides in the mitochondrion intermembrane space. .

Expression: Brain,Cerebellum,Liver,Lymphoma,Osteosarcoma,Peripheral nerve,Semin

Western Blot analysis of MCF7 cells using TNAP Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100

For research use only. Not for use in diagnostic procedures.

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