Talin-1 Polyclonal Antibody

Catalog No.: 

Product Name: Talin-1 Polyclonal Antibody

Catalog No.: ALT5576

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: TLN1

Protein Name: Talin-1

Human Gene ID: 7094

Human Swiss Prot No.: Q9Y490

Other Name: TLN1; KIAA1027; TLN; Talin-1

Subcellular Location: Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell surface . Cell junction, focal adhesion . Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). .

Expression: Aorta,Brain,Embryonic carcinoma,Platelet,

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100

For research use only. Not for use in diagnostic procedures.

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