Product Name: Talin-1 Polyclonal Antibody
Catalog No.: ALT5576
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: TLN1
Protein Name: Talin-1
Human Gene ID: 7094
Human Swiss Prot No.: Q9Y490
Other Name: TLN1; KIAA1027; TLN; Talin-1
Subcellular Location: Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell surface . Cell junction, focal adhesion . Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). .
Expression: Aorta,Brain,Embryonic carcinoma,Platelet,
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
For research use only. Not for use in diagnostic procedures.