Product Name: Survivin (phospho Thr34) Polyclonal Antibody
Catalog No.: ALP0319
Reactivity: Human;Mouse;Rat
Applications: WB;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name: BIRC5
Protein Name: Baculoviral IAP repeat-containing protein 5
Human Gene ID: 332
Human Swiss Prot No.: O15392
Other Name: BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin
Subcellular Location: Cytoplasm . Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Midbody . Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784). .
Expression: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner’s membrane (at protein level) (PubMed:21364656, PubMed:20627126).
Western blot analysis of lysates from HT29 cells treated with EGF 100ug/ml 30′, using Survivin (Phospho-Thr34) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.