Product Name: SHIP-2 Polyclonal Antibody
Catalog No.: ALT5226
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: INPPL1
Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
Human Gene ID: 3636
Human Swiss Prot No.: O15357
Other Name: INPPL1; SHIP2; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Protein 51C; SH2 domain-containing inositol 5′-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2
Subcellular Location: Cytoplasm, cytosol . Cytoplasm, cytoskeleton. Membrane ; Peripheral membrane protein. Cell projection, filopodium . Cell projection, lamellipodium . Nucleus . Nucleus speckle . Translocates to membrane ruffles when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B). Tyrosine phosphorylation may also participate in membrane localization. Insulin specifically stimulates its redistribution from the cytosol to the plasma membrane. Recruited to the membrane following M-CSF stimulation. In activated spreading platelets, localizes with actin at filopodia, lamellipodia and the central actin ring.
Expression: Widely expressed, most prominently in skeletal muscle, heart and brain. Present in platelets. Expressed in transformed myeloid cells and in primary macrophages, but not in peripheral blood monocytes.
Western blot analysis of lysate from HepG2 cells, using INPPL1 Antibody.
For research use only. Not for use in diagnostic procedures.