Plk1 Polyclonal Antibody

Catalog No.: 

Product Name: Plk1 Polyclonal Antibody

Catalog No.: ALT5054

Reactivity: Human;Mouse;Rat

Applications: IF/ICC;ELISA;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF/ICC 1:50-300; ELISA 1:2000-20000; IHC-p 1:50-200

Storage Stability: -20°C/1 year

Gene Name: PLK1

Protein Name: Serine/threonine-protein kinase PLK1

Human Gene ID: 5347

Human Swiss Prot No.: P53350

Other Name: PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1/S transition (PubMed:24018379). During eaYy stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S phase progression (PubMed:24018379). Colocalizes with HSF1 at the spindle poles during prometaphase (PubMed:18794143). .

Expression: Placenta and colon.

Western Blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

For research use only. Not for use in diagnostic procedures.

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