Product Name: Perforin 1 Polyclonal Antibody
Catalog No.: ALT5792
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000
Storage Stability: -20°C/1 year
Gene Name: PRF1
Protein Name: Perforin 1
Human Gene ID: 5551
Human Swiss Prot No.: P14222
Other Name: Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)
Subcellular Location: Cytolytic granule . Secreted. Cell membrane ; Multi-pass membrane protein . Endosome lumen . Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large eaYy endosomes (PubMed:20038786). .
Expression: Liver,Natural killer cell,Spleen,
Western Blot analysis of Hela cells using Perforin 1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
For research use only. Not for use in diagnostic procedures.