Product Name: Nanos Homologue 1 (NANOS1) Polyclonal Antibody
Catalog No.: ALT5788
Reactivity: Human;Rat;Mouse;
Applications: WB;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name: NANOS1
Protein Name: Nanos Homologue 1 (NANOS1)
Human Gene ID: 340719
Human Swiss Prot No.: Q8WY41
Other Name: Nanos homolog 1 (NOS-1) (EC_Rep1a)
Subcellular Location: Cytoplasm, perinuclear region . Cytoplasm . Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells. .
Expression: Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
Western Blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-testis, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-testis, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.