Product Name: Myosin VI Polyclonal Antibody
Catalog No.: ALT5072
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MYO6
Protein Name: Unconventional myosin-VI
Human Gene ID: 4646
Human Swiss Prot No.: Q9UM54
Other Name: MYO6; KIAA0389; Unconventional myosin-VI; Unconventional myosin-6
Subcellular Location: Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Golgi apparatus . Nucleus . Cytoplasm, perinuclear region . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Cell projection, filopodium . Cell projection, ruffle membrane . Cell projection, microvillus . Cytoplasm, cytosol . Also present in endocyctic vesicles (PubMed:16507995). Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage (PubMed:16507995). Recruited into membrane ruffles from cell surface by EGF-stimulation (PubMed:9852149). Colocalizes with DAB2 in clathrin-coated pits/vesicles (PubMed:11967127). Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (By similarity). .; [Isoform 3]: Cytoplasmic vesicle, clathrin-coated vesicle membrane .; [Isoform 4]: Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell projection, ruffle membrane .
Expression: Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.
Western Blot analysis of extracts from Jurkat cells, using Myosin VI Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.