Product Name: IKKβ (phospho Tyr199) Polyclonal Antibody
Catalog No.: ALP0654
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: IKBKB
Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
Human Gene ID: 3551
Human Swiss Prot No.: O14920
Other Name: IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
Subcellular Location: Cytoplasm . Nucleus . Membrane raft . Colocalized with DPP4 in membrane rafts. .
Expression: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-beta (Phospho-Tyr199) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5′, using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.