Product Name: HSP70 Polyclonal Antibody

Catalog No.: ALT5424

Reactivity: Human;Rat;Mouse;

Applications: IF/ICC;WB;IHC-p;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF: 1:50-200 Western Blot: 1/500 – 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: HSPA1A

Protein Name: Heat shock 70 kDa protein 1A/1B

Human Gene ID: 3303/3304

Human Swiss Prot No.: P0DMV8/P0DMV9

Other Name: HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

Subcellular Location: Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Expression: Brain,Cajal-Retzius cell,Embryonic kidney,Epithelium,Fetal

Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of MCF7 cells using HSP70 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100

For research use only. Not for use in diagnostic procedures.

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