Product Name: GSDME n-ternal rabbit pAb
Catalog No.: ALT7990
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution: IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
Storage Stability: -20°C/1 year
Gene Name: GSDME
Protein Name: Gasdermin-E,DFNA5,Dfna5h,EG14210,2310037D07Rik .4932441K13Rik ,ICERE 1,ICERE-1,Deafness, autosomal dominant 5,Non-syndromic hearing impairment protein 5,GSDME-NT
Human Gene ID:
Human Swiss Prot No.: O60443
Subcellular Location: [Gasdermin-E, N-terminal]: Cell membrane ; Multi-pass membrane protein .; [Gasdermin-E]: Cytoplasm, cytosol .
Expression: Expressed in cochlea (PubMed:9771715). Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta (PubMed:9771715).
Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit (Immunoway:RS0002) was diluted at 1:10000
For research use only. Not for use in diagnostic procedures.