GSDME n-ternal rabbit pAb

Catalog No.: 

Product Name: GSDME n-ternal rabbit pAb

Catalog No.: ALT7990

Reactivity: Human;Mouse;Rat

Applications: IF/ICC;WB;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300

Storage Stability: -20°C/1 year

Gene Name: GSDME

Protein Name: Gasdermin-E,DFNA5,Dfna5h,EG14210,2310037D07Rik .4932441K13Rik ,ICERE 1,ICERE-1,Deafness, autosomal dominant 5,Non-syndromic hearing impairment protein 5,GSDME-NT

Human Gene ID:

Human Swiss Prot No.: O60443

Other Name:

Subcellular Location: [Gasdermin-E, N-terminal]: Cell membrane ; Multi-pass membrane protein .; [Gasdermin-E]: Cytoplasm, cytosol .

Expression: Expressed in cochlea (PubMed:9771715). Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta (PubMed:9771715).

Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit (Immunoway:RS0002) was diluted at 1:10000

For research use only. Not for use in diagnostic procedures.

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