Product Name: ERK 1/2 (phospho Tyr204) Polyclonal Antibody
Catalog No.: ALP0101
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MAPK1/MAPK3
Protein Name: Mitogen-activated protein kinase 3
Human Gene ID: 5595/5594
Human Swiss Prot No.: P27361/P28482
Other Name: MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p
Subcellular Location: Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .
Expression: Epithelium,Eye,Hepatoma,Human cervix,Lymph,
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30′, using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.