Product Name: Endoglin Polyclonal Antibody
Catalog No.: ALT5879
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Storage Stability: -20°C/1 year
Gene Name: ENG END
Protein Name: Endoglin (CD antigen CD105)
Human Gene ID: 2022
Human Swiss Prot No.: P17813
Other Name: Endoglin (CD antigen CD105)
Subcellular Location: Cell membrane ; Single-pass type I membrane protein .
Expression: Detected on umbilical veil endothelial cells (PubMed:10625079). Detected in placenta (at protein level) (PubMed:1692830). Detected on endothelial cells (PubMed:1692830).
Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Western blot analysis of lysates from 1) 3T3 , 2) K562 , 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） GAPDH Monoclonal Antibody(2B8) (cat:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.
Western blot analysis of 3T3 KB K562 Hela 293T lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
For research use only. Not for use in diagnostic procedures.