Product Name: Cyclin D1 (phospho Thr286) Polyclonal Antibody
Catalog No.: ALP0297
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CCND1
Protein Name: G1/S-specific cyclin-D1
Human Gene ID: 595
Human Swiss Prot No.: P24385
Other Name: CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
Subcellular Location: Nucleus . Cytoplasm . Nucleus membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. .
Expression: Brain,Placenta,Tongue,
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30′, using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:233(room temperature,ﺈﺈﺊﺌﺎﺎﺐﺒﺔﺔﺖﺘﺚﺜﺞﺠﺢﺤﺦﺨﺪﺪﺬﺬﺮﺮﺰﺰﺲﺴﺶﺸﺺﺼﺾﻀﻂﻄﻆﻈﻊﻌﻎﻐﻒﻔﻖﻘﻚﻜﻞﻠﻢﻤﻦﻬﻮﻮﻰﻰﻲ
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30′, using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
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Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500
For research use only. Not for use in diagnostic procedures.