Product Name: Cdk6 Polyclonal Antibody
Catalog No.: ALT5884
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Storage Stability: -20°C/1 year
Gene Name: CDK6 CDKN6
Protein Name: Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE)
Human Gene ID: 1021
Human Swiss Prot No.: Q00534
Other Name: Cyclin-dependent kinase 6 (EC 2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE)
Subcellular Location: Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). .
Expression: Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200
Western Blot analysis of varius cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS23920 was diluted at 1:10000
For research use only. Not for use in diagnostic procedures.