Product Name: CD19 Polyclonal Antibody

Catalog No.: ALT5580

Reactivity: Human;Rat;Mouse;

Applications: WB;Flow Cyt;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF(paraffin section);ELISA 1:5000-20000

Storage Stability: -20°C/1 year

Gene Name: CD19

Protein Name: B-lymphocyte antigen CD19

Human Gene ID: 930

Human Swiss Prot No.: P15391

Other Name: CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19

Subcellular Location: Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .

Expression: Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).

Western Blot analysis of mouse-kidney mouse-spleen using CD19 Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Western Blot analysis of MOUSE-BRAIN cells using CD19 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

For research use only. Not for use in diagnostic procedures.

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