Product Name: CD11b Polyclonal Antibody
Catalog No.: ALT5923
Reactivity: Human;Rat;Mouse;
Applications: IHC-p;IF(paraffin section);mult-IHC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: IHC-p 1:50-200, ELISA 1:10000-20000
Storage Stability: -20°C/1 year
Gene Name: ITGAM CD11B CR3A
Protein Name: Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)
Human Gene ID: 3684
Human Swiss Prot No.: P11215
Other Name: Integrin alpha-M (CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD antigen CD11b)
Subcellular Location: Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .
Expression: Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).
Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 YT0726 (1/50 dilution), CD11b YT5923 (1/50 dilution), CD14 YM6145 (1/50 dilution),each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Immunoway YS0002, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
For research use only. Not for use in diagnostic procedures.