Product Name: BMP-2 Polyclonal Antibody

Catalog No.: ALT5651

Reactivity: Human;Mouse;Rat

Applications: IF/ICC;WB;IHC-p;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF: 1:50-200 Western Blot: 1/500 – 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: BMP2

Protein Name: Bone morphogenetic protein 2

Human Gene ID: 650

Human Swiss Prot No.: P12643

Other Name: BMP2; BMP2A; Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A; BMP-2A

Subcellular Location: Secreted.

Expression: ParticulaYy abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Immunofluorescence analysis of human-lung tissue. 1,BMP-2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-liver tissue. 1,BMP-2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,BMP-2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of HEPG2-UV cells using BMP-2 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,BMP-2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,BMP-2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

For research use only. Not for use in diagnostic procedures.

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