Product Name: Bag-1 Polyclonal Antibody
Catalog No.: ALT5360
Reactivity: Human;Rat;Mouse;
Applications: WB;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: BAG1
Protein Name: BAG family molecular chaperone regulator 1
Human Gene ID: 573
Human Swiss Prot No.: Q99933
Other Name: BAG1; HAP; BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1
Subcellular Location: [Isoform 1]: Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus.; [Isoform 2]: Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.; [Isoform 4]: Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
Expression: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
Western blot analysis of lysate from AD293 cells, using BAG1 Antibody.
For research use only. Not for use in diagnostic procedures.