Infectious Pancreatic Necrosis Virus (IPNV), Lyophilized
Overview
The Infectious Pancreatic Necrosis Virus (IPNV) Probe-based RT-qPCR Kit (Lyophilized) is a ready-to-use, freeze-dried reagent system for the rapid and sensitive detection of IPNV RNA in biological samples.
This assay utilizes a probe-based real-time RT-qPCR approach to ensure high specificity and accurate quantification of viral load, supporting reliable diagnostics and research in fish health and aquaculture disease monitoring.
Each lyophilized reaction bead is pre-formulated with all essential components — reverse transcriptase, DNA polymerase, dNTPs, optimized buffer, and fluorescent probe mix — ensuring consistent performance and eliminating the need for cold-chain logistics.
Key Features
- Specific and Sensitive: Designed for accurate detection of IPNV RNA using TaqMan-style probe chemistry.
- Lyophilized & Ready-to-Use: Each reaction bead contains all reagents; simply add nuclease-free water and RNA template.
- Room-Temperature Stable: Maintain stability for up to 12 months without refrigeration.
- Fast and Reliable: Compatible with standard real-time PCR instruments for efficient workflows.
- Convenient Format: Ideal for field testing, laboratories with limited cold storage, and routine surveillance programs.
Applications
- Detection and quantification of IPNV in fish tissue, cell culture, or environmental samples
- Aquaculture health monitoring and outbreak investigation
- Research use in virology, epidemiology, and vaccine development
Specifications
| Parameter | Description |
|---|---|
| Detection method | Probe-based real-time RT-qPCR |
| Target | Infectious Pancreatic Necrosis Virus (IPNV) RNA |
| Format | Lyophilized (single-reaction beads) |
| Storage temperature | Room temperature |
| Shelf life | 12 months |
| Intended use | For research use only |
Package Contents
- Lyophilized RT-qPCR reaction beads (pre-aliquoted)
- Nuclease-free rehydration buffer (optional)
- Product manual with detailed instructions
Instructions for Use
- Rehydrate one lyophilized bead with the recommended volume of nuclease-free water.
- Add RNA template (extracted from sample).
- Run on a compatible real-time PCR instrument using the supplied cycling conditions.
- Analyze amplification curves for quantification and interpretation.
Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| IPNV RT-qPCR Kit (Lyophilized) | IPNV-LYO-24 | 24 reactions |
| IPNV RT-qPCR Kit (Lyophilized) | IPNV-LYO-96 | 96 reactions |
Note: For research use only. Not intended for diagnostic or therapeutic applications.