Phosphatidylinositol 3-kinases are a family of intracellular signal transducers involved in cell growth, proliferation, differentiation, motility, apoptosis etc. Activating mutations of PIK3 catalytic subunit are found in several types of cancer, including colorectal cancer, gastric cancer, lung cancer, brain cancer, endometrial cancer, ovarian cancer, breast cancer.
PIK3CA-MMD-PCR Mutation Test Kit enables in one closed-tube reaction detection of four different PIK3CA mutations against a background of wild-type genomic DNA.
The kit allows distinguishing mutations in exon 9 (detected in FAM channel) from mutation in exon 20 (detected in HEX channel).
The GeneFirst OncoPlex® PIK3CA Mutation Test Kit (RUO) is a real-time PCR-based test that detect 4 mutations in the human PIK3CA gene with high sensitivity. The basis for the GeneFirst proprietary technology, MMD, is a combination of specially designed mutation-specific primers, blocker-probe and unique cycling conditions that facilitate selective amplification of multiple mutant alleles – at the same time, blocking amplification from wild type (normal) allele. This allows for the detection of rare mutations against a high background of normal, non-mutated genomic DNA.
