Product Name: ZAP-70 Polyclonal Antibody
Catalog No.: ALT4931
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ZAP70
Protein Name: Tyrosine-protein kinase ZAP-70
Human Gene ID: 7535
Human Swiss Prot No.: P43403
Other Name: ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
Subcellular Location: Cytoplasm . Cell membrane ; Peripheral membrane protein . In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). .
Expression: Expressed in T- and natural killer cells. Also present in eaYy thymocytes and pro/pre B-cells.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZAP-70 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using ZAP-70 Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.