ZAP-70 (phospho Tyr319) Polyclonal Antibody

Catalog No.: 

Product Name: ZAP-70 (phospho Tyr319) Polyclonal Antibody

Catalog No.: ALP0276

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: ZAP70

Protein Name: Tyrosine-protein kinase ZAP-70

Human Gene ID: 7535

Human Swiss Prot No.: P43403

Other Name: ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Subcellular Location: Cytoplasm . Cell membrane ; Peripheral membrane protein . In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). .

Expression: Expressed in T- and natural killer cells. Also present in eaYy thymocytes and pro/pre B-cells.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ZAP-70 (Phospho-Tyr319) Antibody

Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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