Product Name: YAP Polyclonal Antibody
Catalog No.: ALT4924
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: YAP1
Protein Name: Yorkie homolog
Human Gene ID: 10413
Human Swiss Prot No.: P46937
Other Name: YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65
Subcellular Location: Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288, PubMed:20048001). Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288, PubMed:20048001). At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001, PubMed:25849865). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271). Localized mainly to the nucleus in the eaYy stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .
Expression: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Western blot analysis of lysates from HeLa, HepG2, and COLO205 cells, using YAP Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.