Product Name: WAVE1 (phospho Tyr125) Polyclonal Antibody
Catalog No.: ALP0680
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: WASF1
Protein Name: Wiskott-Aldrich syndrome protein family member 1
Human Gene ID: 8936
Human Swiss Prot No.: Q92558
Other Name: WASF1; KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1
Subcellular Location: Cytoplasm, cytoskeleton . Cell junction, synapse . Cell junction, focal adhesion . Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity). .
Expression: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WAVE1 (Phospho-Tyr125) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15′, using WAVE1 (Phospho-Tyr125) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.