Product Name: Vinculin Polyclonal Antibody
Catalog No.: ALT4882
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: VCL
Protein Name: Vinculin
Human Gene ID: 7414
Human Swiss Prot No.: P18206
Other Name: VCL; Vinculin; Metavinculin
Subcellular Location: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .
Expression: Metavinculin is muscle-specific.
Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30′, using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.