Product Name: Trk A (phospho Tyr701) Polyclonal Antibody
Catalog No.: ALP0777
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: NTRK1
Protein Name: High affinity nerve growth factor receptor
Human Gene ID: 4914
Human Swiss Prot No.: P04629
Other Name: NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;
Subcellular Location: Cell membrane ; Single-pass type I membrane protein . EaYy endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Recycling endosome membrane ; Single-pass type I membrane protein . Rapidly internalized after NGF binding (PubMed:1281417). Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and eaYy endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes. .
Expression: Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk A (Phospho-Tyr701) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using Trk A (Phospho-Tyr701) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from mouse brain, using Trk A (Phospho-Tyr701) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.