Product Name: Topo IIα (phospho Ser1525) Polyclonal Antibody
Catalog No.: ALP0836
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: TOP2A
Protein Name: DNA topoisomerase 2-alpha
Human Gene ID: 7153
Human Swiss Prot No.: P11388
Other Name: TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme
Subcellular Location: Cytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus .
Expression: Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056).
Immunohistochemistry analysis of paraffin-embedded human brain, using TOP2A (Phospho-Ser1525) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 5′, using TOP2A (Phospho-Ser1525) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.