Tie-2 (phospho Tyr992) Polyclonal Antibody

Catalog No.: 

Product Name: Tie-2 (phospho Tyr992) Polyclonal Antibody

Catalog No.: ALP0437

Reactivity: Human;Mouse

Applications: WB; ELISA;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

Storage Stability: -20°C/1 year

Gene Name: TEK

Protein Name: Angiopoietin-1 receptor

Human Gene ID: 7010

Human Swiss Prot No.: Q02763

Other Name: TEK; TIE2; VMCM; VMCM1; Angiopoietin-1 receptor; Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor

Subcellular Location: Cell membrane ; Single-pass type I membrane protein. Cell junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton. Secreted . Recruited to cell-cell contacts in quiescent endothelial cells (PubMed:18425120, PubMed:18425119). Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted (PubMed:11806244). .

Expression: Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.


Western Blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody


Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).


Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).


Western Blot analysis of mouse lung , mouse liver ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

For research use only. Not for use in diagnostic procedures.

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