Product Name: Stat1 (phospho Ser727) Polyclonal Antibody
Catalog No.: ALP0842
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: STAT1
Protein Name: Signal transducer and activator of transcription 1-alpha/beta
Human Gene ID: 6772
Human Swiss Prot No.: P42224
Other Name: STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
Subcellular Location: Cytoplasm . Nucleus . Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4 (PubMed:15322115). Monomethylation at Lys-525 is required for phosphorylation at Tyr-701 and translocation into the nucleus (PubMed:28753426). Translocates into the nucleus in response to interferon-beta stimulation (PubMed:26479788). .
Expression: B-cell,Brain,Retina,Testis,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT1 (Phospho-Ser727) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.