Smad3 (phospho Ser213) Polyclonal Antibody

Catalog No.: 

Product Name: Smad3 (phospho Ser213) Polyclonal Antibody

Catalog No.: ALP0364

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: SMAD3

Protein Name: Mothers against decapentaplegic homolog 3

Human Gene ID: 4088

Human Swiss Prot No.: P84022

Other Name: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Subcellular Location: Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the eaYy stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .

Expression: Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser213) Antibody


Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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