Product Name: Smad2 (phospho Thr220) Polyclonal Antibody
Catalog No.: ALP1185
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000,Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000,WB 1:500-2000
Storage Stability: -20°C/1 year
Gene Name: SMAD2
Protein Name: Mothers against decapentaplegic homolog 2
Human Gene ID: 4087
Human Swiss Prot No.: Q15796
Other Name: SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Subcellular Location: Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the eaYy stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .
Expression: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) Antibody. The picture on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.