Product Name: Smad1 (phospho Ser465) Polyclonal Antibody
Catalog No.: ALP0583
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: SMAD1
Protein Name: Mothers against decapentaplegic homolog 1
Human Gene ID: 4086
Human Swiss Prot No.: Q15797
Other Name: SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein
Subcellular Location: Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). .
Expression: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad1 (Phospho-Ser465) Antibody
Immunohistochemistry analysis of paraffin-embedded human heart, using Smad1 (Phospho-Ser465) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with Serum 10% 15′, using Smad1 (Phospho-Ser465) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.