Shc (phospho Tyr427) Polyclonal Antibody

Catalog No.: 

Product Name: Shc (phospho Tyr427) Polyclonal Antibody

Catalog No.: ALP0579

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: SHC1

Protein Name: SHC-transforming protein 1

Human Gene ID: 6464

Human Swiss Prot No.: P29353

Other Name: SHC1; SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1

Subcellular Location: Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix . Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; [Isoform p66Shc]: Mitochondrion . In case of oxidative conditions, phosphorylation at ‘Ser-36’ of isoform p66Shc, leads to mitochondrial accumulation. .

Expression: Widely expressed. Expressed in neural stem cells but absent in mature neurons.

Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5′, using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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