Product Name: RN112 rabbit pAb
Catalog No.: ALT6529
Reactivity: Human; Mouse;Rat
Applications: WB;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300
Storage Stability: -20°C/1 year
Gene Name: RNF112 BFP ZNF179
Protein Name: RN112
Human Gene ID: 7732
Human Swiss Prot No.: Q9ULX5
Other Name:
Subcellular Location: Membrane ; Multi-pass membrane protein . Membrane ; Peripheral membrane protein . Cytoplasm . Nucleus . Nucleus, nuclear body . Nucleus, nucleoplasm . Endosome . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle . Cell junction, synapse, postsynaptic density . Perikaryon . Cell projection, neuron projection . Predominantly in the nucleus, but some amounts were also found in the cytoplasm. Oxidative stress stimulates its shuttling from the cytoplasm into the nucleus. Recruited to nuclear bodies via its interaction with ZBTB16. Localizes to the cell soma and neuritis and only slightly to the nucleus in the neurons of most brain areas. .
Expression: Predominantly expressed in brain (PubMed:10574464). Decreased expression in glioma brain tumors as compared to normal brains (at protein level) (PubMed:28684796).
Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.