Product Name: Rb (Acetyl-K873/K874) Polyclonal Antibody
Catalog No.: ALK0092
Reactivity: Human:K873+K874;Mouse:K866+K867;Rat:K865+K866
Applications: WB;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300
Storage Stability: -20°C/1 year
Gene Name: RB1
Protein Name: Rb
Human Gene ID: 5925
Human Swiss Prot No.: P06400
Other Name: Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
Subcellular Location: Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. .
Expression: Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255).
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.