Product Name: RASF5 rabbit pAb
Catalog No.: ALT6737
Reactivity: Human; Mouse;Rat
Applications: WB;IF/ICC;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
Storage Stability: -20°C/1 year
Gene Name: RASSF5 NORE1 RAPL
Protein Name: RASF5
Human Gene ID: 83593
Human Swiss Prot No.: Q8WWW0
Other Name:
Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
Expression: Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.