Product Name: Raf-B (phospho Thr753) Polyclonal Antibody
Catalog No.: ALP0461
Reactivity: Human;Mouse;Rat
Applications: WB;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name: BRAF
Protein Name: Serine/threonine-protein kinase B-raf
Human Gene ID: 673
Human Swiss Prot No.: P15056
Other Name: BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Subcellular Location: Nucleus . Cytoplasm . Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .
Expression: Brain and testis.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Raf (Phospho-Thr753) Antibody
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30′, using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.