Raf-B (phospho Ser446) Polyclonal Antibody

Catalog No.: 

Product Name: Raf-B (phospho Ser446) Polyclonal Antibody

Catalog No.: ALP0780

Reactivity: Human;Mouse

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: BRAF

Protein Name: Serine/threonine-protein kinase B-raf

Human Gene ID: 673

Human Swiss Prot No.: P15056

Other Name: BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Subcellular Location: Nucleus . Cytoplasm . Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .

Expression: Brain and testis.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-RAF (Phospho-Ser446) Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30′, using B-RAF (Phospho-Ser446) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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