Raf-1 (phospho Ser259) Polyclonal Antibody

Catalog No.: 

Product Name: Raf-1 (phospho Ser259) Polyclonal Antibody

Catalog No.: ALP0239

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: RAF1

Protein Name: RAF proto-oncogene serine/threonine-protein kinase

Human Gene ID: 5895

Human Swiss Prot No.: P04049

Other Name: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Subcellular Location: Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.

Expression: In skeletal muscle, isoform 1 is more abundant than isoform 2.


Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C-RAF (Phospho-Ser259) Antibody.


Western blot analysis of lysates from K562 cells, using C-RAF (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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