Product Name: PYK2 (phospho Tyr580) Polyclonal Antibody
Catalog No.: ALP0610
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: PTK2B
Protein Name: Protein-tyrosine kinase 2-beta
Human Gene ID: 2185
Human Swiss Prot No.: Q14289
Other Name: PTK2B; FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Pro
Subcellular Location: Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cell cortex. Nucleus. Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.
Expression: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PYK2 (Phospho-Tyr580) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells, using PYK2 (Phospho-Tyr580) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.