PRAS40 (phospho Thr246) Polyclonal Antibody

Catalog No.: 

Product Name: PRAS40 (phospho Thr246) Polyclonal Antibody

Catalog No.: ALP0302

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: AKT1S1

Protein Name: Proline-rich AKT1 substrate 1

Human Gene ID: 84335

Human Swiss Prot No.: Q96B36

Other Name: AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

Subcellular Location: Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .

Expression: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt1 S1 (Phospho-Thr246) Antibody

Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30′, using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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