Product Name: PRAS40 (phospho Thr246) Polyclonal Antibody
Catalog No.: ALP0302
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: AKT1S1
Protein Name: Proline-rich AKT1 substrate 1
Human Gene ID: 84335
Human Swiss Prot No.: Q96B36
Other Name: AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Subcellular Location: Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
Expression: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt1 S1 (Phospho-Thr246) Antibody
Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30′, using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.