Product Name: PR (phospho Ser400) Polyclonal Antibody
Catalog No.: ALP0532
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300
Storage Stability: -20°C/1 year
Gene Name: PGR
Protein Name: Progesterone receptor
Human Gene ID: 5241
Human Swiss Prot No.: P06401
Other Name: PGR; NR3C3; Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3
Subcellular Location: Nucleus. Cytoplasm. Nucleoplasmic shuttling is both hormone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.; [Isoform A]: Nucleus. Cytoplasm. Mainly nuclear.; [Isoform 4]: Mitochondrion outer membrane .
Expression: In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Progesterone Receptor (Phospho-Ser400) Antibody
Immunofluorescence analysis of A549 cells, using Progesterone Receptor (Phospho-Ser400) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with heat shock, using Progesterone Receptor (Phospho-Ser400) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.